Last data update: 24 January 2024 16:39 CET
Plasmid name: pUC21:synthCandidaBAX (LMBP 4436)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4436.gb
(View with Genome Compiler) p4436.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse Bcl2-associated X cDNA (Bax, BAX); Candida albicans codon adapted sequence (CaBAX) |
| Promoter: | Escherichia coli lac operon promoter; modified Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Bacteriophage M13 origin; with deletion |
| Host range: | Escherichia coli |
| Parental clone: | pUC21 |
| Further information: | The plasmid contains the Candida albicans codon adapted coding sequence of the mouse Bcl2-associated X cDNA (CaBAX), inserted into the polylinker of pUC21. The plasmid is non-transferable and has a high copy number. The strength of the lac promoter is reduced by increasing the distance between the -10 and -35 elements: the HpaII site was filled in, creating a SacII site. The yield of plasmid ssDNA is improved by a deletion within the M13 minus ori (from nucleotide 5644 to 5721 of the M13 genome). When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727208.1. The nucleotide sequence of pUC21 corresponds with the Genbank accession number AF223641.1. |
| EMBL Accession number: | AF223641.1, view at EMBL, GenBank, DDBJ LT727208.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 27/09/2005 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BglII and HindIII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Contreras(1) (2). It was constructed by I. Eberhardt(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | De Smet et al., Yeast 21 (2004), 1325-1334 [PMID: 15565645] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUC21:synthCandidaBAX (LMBP 4436) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Contreras and was published in De Smet et al., 2004. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.