Last data update: 24 January 2024 16:39 CET
Plasmid name: pUD1 (LMBP 4355)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
pud1.gb
(View with Genome Compiler) pud1.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Saccharomyces cerevisiae actin promoter |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae URA3; auxotrophic Methotrexate (MTX) |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae autonomously replicating sequence (ARS1) Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-) |
| Parental clone: | pUD0; pEX-6 |
| Further information: | This plasmid is useful for dominant or auxotrophic integrative transformation of Saccharomyces cerevisiae. The plasmid contains the 2μ origin region (1467 bp HindII fragment). The nucleotide sequence of the actin promoter is not known. |
| EMBL Accession number: | - |
| Latest sequence update: | 24/04/2001 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AlwNI, BglI, EcoRI, NcoI, PstI and SphI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Contreras(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Zhu et al., Biotechnology 3 (1985), 451-456 |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pUD1 (LMBP 4355) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Contreras and was published in Zhu et al., 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.