Last data update: 24 January 2024 16:39 CET
Plasmid name: pULG332 (LMBP 3230)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
pulg332.gb
(View with Genome Compiler) pulg332.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Moraxella strain TA144 lipase 2 genomic DNA (lip2) |
| Promoter: | Moraxella strain TA144 lipase2 promoter (lip2) Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Moraxella strain TA144 lipase 2 gene (lip2) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pSP73 |
| Further information: | The construction of this plasmid is described in Feller et al., Gene 102 (1991), 111-115. The plasmid numbering in this publication is not correct: plasmid pULG332 is in fact pULG333 (insert size 2.1 kb). pULG332 contains an additional 1.5 kb DNA fragment downstream from the last 3' EcoRV site. pULG333 (no longer available) has been constructed by inserting 1.65 and 0.45 kb EcoRV fragments from pULG332 in pSP73 (digested with EcoRV and PvuII, dephosphorylated) and selecting the lipase positive clones on LB plates + 0.5% tributyrine (Dr. G. Feller, personal communication). Since the nucleotide sequence of the 1.5 kb genomic lip2 DNA fragment could not be completely traced back, there is uncertainty as to the cutting frequency of the restriction enzymes, except for the sites that were analysed in the authenticity test. The nucleotide sequence of the parental clone pSP73 corresponds with the EMBL Nucleotide Sequence Database (Release 36; Accession number X65333). The nucleotide sequence of the Moraxella sp. lip2 gene corresponds with the EMBL Nucleotide Sequence Database (Release 35; Accession number X53868). |
| EMBL Accession number: | X53868, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 24/01/1995 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI and EcoRV. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr G. Feller(1). (1) Laboratoire de Biochimie, Universitδ de Liège, Institut de Chimie B6, Sart Tilman, Liège, Belgium |
| Plasmid reference: | Feller et al., Gene 102 (1991), 111-115 [PMID: 1864500] |
| Related plasmid reference: | Feller et al., DNA Cell Biol. 10 (1991), 381-388 [PMID: 1907455] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 RR1ZΔM15 |
| Host reference: | Altieri et al., J. Bacteriol. 168 (1986), 648-654 [PMID: 2946661] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pULG332 (LMBP 3230) is available at BCCM/GeneCorner. This plasmid was deposited by Dr G. Feller and was published in Feller et al., 1991. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.