Last data update: 24 January 2024 16:39 CET
Plasmid name: pURgHIL22 (LMBP 1012)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p1012.gb
(View with Genome Compiler) p1012.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interleukin 2 genomic DNA (IL2, IL-2, TCGF, GeneID 3558); exon 3 and 4 |
| Promoter: | Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pUR250 |
| Further information: | pURgHIL22 contains the third and fourth exon of the human interleukin 2 gene (hIL2), part of the second intron (approx. 1000 bp of a total of 2300 bp) and the third intron. The sequence of the genomic hIL2 insert was obtained from Genbank 'Locus: HUMIL2'; it differs in several nucleotides from the incompletely determined hIL2 sequence as described by Degrave et al. (1983); more differences are to be expected, since the XbaI site at position 3220 (i.e. almost coinciding with the unique BglII site in intron 3) in the genomic hIL2 sequence obtained from Genbank and described by Fujita et al. (1983) could not be detected experimentally in the clone pURgHIL22 of Degrave et al. (1983). Note that, because of the computer plot programme, this hypothetical XbaI site is indicated on the circular map. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727567.1. Name mentioned in Degrave et al. (1983) and in Devos et al. (1983) is pUR-gHil2-2. |
| EMBL Accession number: | J00264, view at EMBL, GenBank, DDBJ LT727567.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 06/12/2000 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindIII, SalI and XbaI. pUR250 was mutagenised in vivo and not resequenced; it may therefore contain unknown mutations. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Devos et al., Nucleic Acids Res. 11 (1983), 4307-4323 [PMID: 6306584] |
| Related plasmid reference: | Fujita et al., Proc. Natl. Acad. Sci. U.S.A. 80 (1983), 7437-7441 [PMID: 6324170] Degrave et al., EMBO J. 2 (1983), 2349-2353 [PMID: 6321163] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 RR1ZΔM15 |
| Host reference: | Altieri et al., J. Bacteriol. 168 (1986), 648-654 [PMID: 2946661] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pURgHIL22 (LMBP 1012) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Devos et al., 1983. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.