Last data update: 24 January 2024 16:39 CET
Plasmid name: pYES2-10His-rAQP8 (LMBP 11182)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p11182.gb |
| Sequence analysis results Genecorner: |
NGS: h02-gc-dec2018.fasta |
| Cloned DNA: |
Rattus norvegicus aquaporin 8 cDNA (Aqp8, GeneID 29172) Histidine tag (His-tag); N-terminal |
| Promoter: | Saccharomyces cerevisiae galactokinase promoter (GAL1) Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Ampicillin (amp) URA3; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-) |
| Parental clone: | pYES2 |
| Further information: | The plasmid was constructed by cloning the rat Aqp8 coding sequence into the pYES2 vector. This plasmid is designed for high-level expression in Saccharomyces cerevisiae. The plasmid contains the GAL1 promoter for inducible expression of genes by galactose and repression by glucose. The nucleotide sequence of the rat Aqp8 coding sequence corresponds with the Genbank accession number XM_006230187.3. |
| EMBL Accession number: | XM_006230187.3, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 14/03/2019 |
| Authenticity test: | This plasmid has been fully sequenced. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr G. Calamita (1). It was constructed by D. Frascaria (1). (1) Department of Biosciences, Biotechnologies and Biopharmaceutics, University of Bari "Aldo Moro", Bari, Italy |
| Plasmid reference: | Liu et al., Biol. Cell 98 (2006), 153-161 [PMID: 15948717] [DOI: 10.1042/BC20050026] |
| Related plasmid reference: | Soria et al., Biochem. Biophys. Res. Commun. 393 (2010), 217-221 [PMID: 20132793] [DOI: 10.1016/j.bbrc.2010.01.104] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pYES2-10His-rAQP8 (LMBP 11182) is available at BCCM/GeneCorner. The plasmid was deposited by Dr G. Calamita and was published in Liu et al., 2006. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.