Last data update: 24 January 2024 16:39 CET
Plasmid name: pcDNA3-F-HLH-p76-MALT1x-VSV-HA (LMBP 9824)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, paracaspase 1, GeneID 10892); fragment FLAG epitope tag; N-terminal HLH dimerization domain; N-terminal Vesicular stomatitis virus glycoprotein (VSV-G) epitope Influenza HA epitope encoding the haemagglutinin tagging peptide; C-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Simian virus 40 early promoter (SV40 early) Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pcDNA3-F-HLH-Ig2-VSV-MALT1C-HA |
| Further information: | The plasmid was constructed by removing the Ig2 from pcDNA3-F-HLH-Ig2-VSV-MALT1C-HA as a BamHI/XhoI fragment, and replacing it with a BglII-KpnI-BamHI-Ig1-Ig2-XhoI fragment. The resulting HLH-p76 MALT1 is in frame with the VSV-MALT1C-HA. The plasmid is intended for functional characterization of mutations and (auto)processing events in human MALT1, via strategically placed FLAG, VSV and HA epitope tags. The plasmid contains the ETV6 helix-loop-helix domain (HLH) which is a dimerization domain, shown to strongly activate human MALT1. The immunoglobulin-like domains Ig1 and Ig2 of hMALT1 contribute to the oligomerization of the caspase-like domain and help with the correct dimerization. The HLH domain can be deleted using KpnI. Other name of the plasmid is pCDNA3-F-HLH-p76-MALT1x-vsv-WT-HA. |
| EMBL Accession number: | - |
| Latest sequence update: | 29/10/2019 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2) and Dr J. Staal(1) (2). (1) Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pcDNA3-F-HLH-p76-MALT1x-VSV-HA (LMBP 9824) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and Dr J. Staal . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.