Last data update: 24 January 2024 16:39 CET
Plasmid name: piCA3N (LMBP 6251)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | - |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Phage P22 repressor gene (c2) Escherichia coli DNA-binding transcriptional repressor LacI cDNA (lacI, GeneID 945007) Phage P22 antirepressor gene (ant) Escherichia coli plasmid pSC101 replication protein A (repA) Phage λ regulatory protein cDNA (cro) |
Promoter: | Escherichia coli lac repressor promoter (lacI) Phage T5 N25 promoter with two lac operator sequences (N25/O2) Phage 22 major rightward promoter (P22 PR) Phage 22 promoter for repressor maintenance (P22 PM) |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Kanamycin (kan) |
Replicon: | Plasmid pSC101 origin derived from R6-5 |
Copy number: | Low copy number |
Host range: | Escherichia coli |
Parental clone: | piCA3 |
Further information: | The plasmid was constructed by cloning the phage λ cro peptide into the piCA3 vector, between the lacI and the P22 c2 coding sequences. - |
EMBL Accession number: | - |
Latest sequence update: | 26/03/2009 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by J. Haustraete(1) (2). (1) Protein Core, VIB-UGent Center for Inflammation Research, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
piCA3N (LMBP 6251) is available at BCCM/GeneCorner. This plasmid was deposited by J. Haustraete . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.