Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAD/CaHygB (LMBP 11191)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p11191.gb |
| Sequence analysis results Genecorner: |
NGS: h09-gc-dec2019.fasta |
| Cloned DNA: |
Phage P1 cre gene encoding recombinase; with Kozak consensus sequence |
| Promoter: | Candida albicans elongation factor 1-alpha 2 promoter (TEF2) Candida albicans alpha-glucosidase promoter (MAL2) |
| Ribosome binding site: |
- |
| Terminator: | Candida albicans ATP-dependent 6-phosphofructokinase subunit beta terminator (PFK2) |
| Selection marker: | Hygromycin (hyg) Ampicillin (amp) Candida albicans ARG4; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Candida albicans |
| Parental clone: | pCAD |
| Further information: | The plasmid was constructed by replacing the MET3 promoter- Cre-URA3 cassette of pCAD with a MAL2 promoter-Cre-HygB cassette. The plasmid contains a Cre gene under the control of the MAL2 promoter, meant for eviction of the HIS1-ARG4-HIS1 gene disruption cassette from p416-HAH2, p416-HAH2-PTET and p416-HAH2-MET3. The nucleotide sequence of the plasmid corresponds with the EMBL Nucleotide Sequence Database accession number MF048862.1. |
| EMBL Accession number: | MF048862.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 26/03/2019 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr K. Ganesan(1). It was constructed by Dr S. Bijlani(1). (1) CSIR Institute of Microbial Technology, Chandigarh, India |
| Plasmid reference: | - |
| Related plasmid reference: | Dennison et al., Fungal Genet. Biol. 42 (2005), 737-748 [PMID: 16043373] [DOI: 10.1016/j.fgb.2005.05.006] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAD/CaHygB (LMBP 11191) is available at BCCM/GeneCorner. The plasmid was deposited by Dr K. Ganesan. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.