Last data update: 24 January 2024 16:39 CET
Plasmid name: pENTR/D-TOPO-hPCDH17-DN-HA (LMBP 12579)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human protocadherin 17 cDNA (PCDH17, PCDH68, PCH68, GeneID 27253); fragment Influenza HA epitope encoding the haemagglutinin tagging peptide; C-terminal |
| Promoter: | Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pENTR/D-TOPO-hPCDH17 |
| Further information: | The plasmid was constructed by replacing the cytoplasmic tail of human PCDH17 in pENTR/D-TOPO-hPCDH17 with a HA-tag. This plasmid encodes a dominant negative form of human PCDH17. Other name of the plasmid is pENTRDtopo-hPCDH17-HA DN cl3. |
| EMBL Accession number: | - |
| Latest sequence update: | 11/10/2021 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by K. Staes(1)(2) and Prof. Dr F. Van Roy(1)(2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pENTR/D-TOPO-hPCDH17-DN-HA (LMBP 12579) is available at BCCM/GeneCorner. The plasmid was deposited by K. Staes and Prof. Dr F. Van Roy. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.