Last data update: 24 January 2024 16:39 CET
Plasmid name: pPTK109_P2-4_sfGFP (LMBP 13775)
New search | Print data sheet |
Price category: | Cat. 3 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p13775.gb
(View with Genome Compiler) p13775.txt p13775.pdf |
Sequence analysis results Genecorner: |
Sanger: ef32708316.fasta Sanger: ef32708315.fasta NGS: 13775-ps-gc-sep2023.fasta Sanger: .fasta |
Cloned DNA: |
Synthetic green fluorescent protein DNA (GFP); superfolder GFP (sfGFP) |
Promoter: | Escherichia coli glycerol-3-phosphate transporter promoter (glpT) |
Ribosome binding site: |
Ribosome binding site (RBS); synthetic sequence |
Terminator: | Escherichia coli rrnB operon T1 terminator Phage T7 early transcription terminator (T7Te) |
Selection marker: | Chloramphenicol (cam) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli Pichia pastoris; as part of the OPENPichia plasmid kit |
Parental clone: | pPTK_0 |
Further information: | The plasmid was constructed by cloning the synthetic sfGFP coding sequence, codon optimised for P. pastoris, in the synthetic pPTK_0 vector. This plasmid contains a fluorescent marker cassette intended for cloning into a multigene acceptor plasmid. It thereby acts as a combination of OPENPichia parts 2, 3 and 4 for the OPENPichia modular cloning technology. Upon BsaI restriction, it leaves appropriate overhangs to combine with OPENPichia part 1 and OPENPichia part 5. Other name of the plasmid is pPTK109. |
EMBL Accession number: | - |
Latest sequence update: | 18/04/2023 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: NcoI and PvuII. The region containing the sfGFP coding sequence, including promoter and terminator sequences, and most of the pMB1 ori and the chloramphenicol resistance gene, have been sequenced by BCCM/GeneCorner. The plasmid has also been fully sequenced. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by S. Vanmarcke(1)(2) and Prof. Dr N. Callewaert(1)(2). It was constructed by Dr K. Vandewalle(1)(2) and Dr R. Vanluchene(1)(2). (1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium (2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
Plasmid reference: | Claes et al., Nature (2024) [PMID: 38443579] [DOI: 10.1038/s41564-023-01574-w] |
Restricted distribution: | - BCCM MTA adapted for OPENPichia - RECIPIENT agrees to refer to the 'Plasmid reference' in the first publication making use of the MATERIAL. - When appropriate in accordance with academic customs, RECIPIENT agrees to include the depositor(s) as co-author(s) in the first publication making use of the MATERIAL. - When making use of the MATERIAL, RECIPIENT agrees to refer to ‘OPENPichia’ in the Materials and Methods section and in the acknowledgments of all publications, as well as when launching commercial products. - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department. |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5αT1R |
Host reference: | - |
Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
Cultivation medium: | LB-Lennox + chloramphenicol (34 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPTK109_P2-4_sfGFP (LMBP 13775) is available at BCCM/GeneCorner. This plasmid was deposited by S. Vanmarcke and Prof. Dr N. Callewaert and was published in Claes et al., 2024. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.