Last data update: 24 January 2024 16:39 CET
Plasmid name: pEF6-mTRAF2-cMyc (LMBP 6140)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p6140.gb
(View with Genome Compiler) p6140.txt p6140.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse TNF receptor-associated factor 2 cDNA (Traf2, GeneID 22030) Myc epitope; C-terminal Histidine tag (His-tag); C-terminal |
| Promoter: | Human elongation factor 1α promoter (EF1α) Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Synthetic prokaryotic EM7 promoter Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Blasticidin (bsd) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli; preferably recombination-deficient and endonuclease A deficient strains Mammalian cells; SV40 permissive cells |
| Parental clone: | pEF6/E-Myc-HisA; pRK5-mTRAF2 |
| Further information: | The plasmid was constructed by PCR amplifying the mouse TRAF2 coding sequence from pRK5-mTRAF2, and cloning it as a BamHI/XbaI fragment into the BamHI/XbaI opened pEF6/E-Myc-HisA vector. - The nucleotide sequence of the mouse TRAF2 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number L35303.1. The nucleotide sequence of the parental plasmid pEF6/E-Myc-HisA corresponds with the EMBL Nucleotide Sequence Database accession number LT727183.1. |
| EMBL Accession number: | L35303.1, view at EMBL, GenBank, DDBJ LT727183.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 09/10/2013 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI/XbaI, BglII, EcoRI, NcoI and NotI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEF6-mTRAF2-cMyc (LMBP 6140) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.