Last data update: 24 January 2024 16:39 CET
Plasmid name: pEXPR-E-slrP (LMBP 6729)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6729.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Salmonella enterica serovar Typhimurium LT2 leucine-rich repeat protein cDNA (slrP) E-tag; N-terminal |
| Promoter: | Simian cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage T7 gene 10 promoter (T7g10) Phage T3 promoter Phage SP6 promoter Escherichia coli lac operon promoter; mutant (lacUV5) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Mammalian cells Avian cells |
| Parental clone: | pDEST-Etag; pENTR201-slrP |
| Further information: | This Gateway expression vector was constructed via Gateway recombination, cloning the slrP cDNA from pENTR201-slrP into the pDEST-Etag vector. Protein expression of this vector in HEK293T cells was tested at GeneCorner. |
| EMBL Accession number: | AE006468, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 06/08/2010 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HindIII/SacI, NarI, SacII and XhoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by W. De Schamphelaire(1) and Prof. Dr R. Beyaert(1). (1) BCCM/GeneCorner, Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEXPR-E-slrP (LMBP 6729) is available at BCCM/GeneCorner. This plasmid was deposited by W. De Schamphelaire and Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.