Last data update: 24 January 2024 16:39 CET
Plasmid name: pIB4 (LMBP 5770)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5770.gb
(View with Genome Compiler) p5770.txt p5770.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Pichia pastoris alcohol oxidase 1 promoter (AOX1) |
| Ribosome binding site: |
- |
| Terminator: | Pichia pastoris alcohol oxidase 1 terminator (AOX1) |
| Selection marker: | Ampicillin (amp) HIS4; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Pichia pastoris; his4(-), integrative |
| Parental clone: | pIB1; pHIL-D2 |
| Further information: | pIB4 is an integrating vector for regulated gene expression in P. pastoris under the control of the strong, highly-inducible P. pastoris AOX1 promoter. The plasmid contains a modified pUC19 polylinker: the XbaI and SalI sites are replaced by SpeI and XhoI sites respectively. pIB4 contains a modified P. pastoris HIS4 gene: the internal BamHI site is removed and the KpnI sites are eliminated because of two silent point mutations. The nucleotide sequence of pIB4 corresponds with the EMBL Nucleotide Sequence Database accession number AF027961.1. |
| EMBL Accession number: | AF027961.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 16/03/2009 |
| Sequence detail: | Sequence of the modified pUC19 polylinker: GAA.TTC.GAG.CTC.GGT.ACC.CGG.GGA.TCC.ACT.AGT.CTC.GAG.CTG.CAG.GCA.TGC.AAG.CTT EcoRI SacI KpnI SmaI BamHI SpeI XhoI PstI SphI HindIII |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BglI, HaeII/HincII and PvuII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr B. Glick(1). (1) Department of Molecular Genetics and Cell Biology, University of Chicago, USA |
| Plasmid reference: | Sears et al., Yeast 14 (1998), 783-790 [PMID: 9675822] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pIB4 (LMBP 5770) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr B. Glick and was published in Sears et al., 1998. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.