Last data update: 24 January 2024 16:39 CET
Plasmid name: pJB3 (LMBP 3042)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3042.gb
(View with Genome Compiler) p3042.txt p3042.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli lac Z gene (lacZ); α part Broad-host-range plasmid RK2 trans-acting replication initiator protein DNA (trfA) |
| Promoter: | Escherichia coli lac operon promoter Transposon Tn5 neomycin resistance gene promoter (neo) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Broad-host-range plasmid RK2 vegetative replication origin Broad-host-range plasmid RK2/RP4 origin of transfer (oriT) |
| Host range: | Escherichia coli Gram-negative bacterial strains |
| Parental clone: | pJB7; pKH3 |
| Further information: | This IncP plasmid is useful as a broad-host-range cloning vector. The plasmid RK2 trfA gene encodes two polypeptide products P382 and P285 of 382 and 285 amino acids respectively, using a different initiation codon within the same reading frame. The resulting protein is essential for vegetative replication. Introduce the plasmid by electroporation, or through conjugation, using plasmid RK2/RP4 transfer functions (provided in trans by RK2/RP4, RK2/RP4-derived vectors, other replicons carrying RK2/RP4 transfer functions (e.g. pRK2073) or strains expressing the tra genes of RK2/RP4). When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of the plasmid corresponds with the complemented EMBL Nucleotide Sequence Database accession number U73899.1, starting at another position. The nucleotide sequence of the RK2 replication origin corresponds with the EMBL Nucleotide Sequence Database accession number J01780.1. |
| EMBL Accession number: | U73899.1, view at EMBL, GenBank, DDBJ J01780.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 02/05/1994 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI, EcoRI, NarI/SspI, PvuI, PvuII, RsaI and XmnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr S. Valla(1). (1) Unigen Center for Molecular Biology, Norwegian University of Science and Technology, Trondheim, Norway. |
| Plasmid reference: | Blatny et al., Appl. Environ. Microbiol. 63 (1997), 370-379 [PMID: 9023917] |
| Related plasmid reference: | Remaut et al., in 'Biotechnological Applications of Cold-Adapted Organisms', Springer (1999), 1-16 [ISSN/ISBN: 3642636632] [DOI: 10.1007/978-3-642-58607-1] Smith et al., J. Mol. Biol. 175 (1984), 251-262 [PMID: 6374158] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pJB3 (LMBP 3042) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Valla and was published in Blatny et al., 1997. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.