Last data update: 24 January 2024 16:39 CET
Plasmid name: pPLaACR26 (LMBP 311)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p311.gb
(View with Genome Compiler) p311.txt p311.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Phage MS2 coat protein gene Phage MS2 polymerase gene Phage MS2 assembly protein gene Phage MS2 lysis protein gene |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage MS2 coat protein gene Ribosome binding site (RBS) of the phage MS2 polymerase gene Ribosome binding site (RBS) of the phage MS2 assembly protein gene |
| Terminator: | - |
| Selection marker: | Kanamycin (kan) |
| Replicon: | Escherichia coli plasmid ColE1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pPLa2311; pMS27 |
| Further information: | This plasmid contains the coding information of phage MS2, in a sense orientation relative to the λ PL promoter. The exact length of the dA/dT-tails is not known. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727439.1. Other name of the plasmid is pSRKMS26. |
| EMBL Accession number: | LT727439.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 25/03/1992 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BanII, EcoRI/PstI, HindIII and XhoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Remaut et al., EMBO J. 1 (1982), 205-209 [PMID: 16453413] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 ΔH1Δtrp |
| Host reference: | Bernard et al., Gene 5 (1979), 59-76 [PMID: 372049] |
| Related host reference: | Remaut et al., Gene 15 (1981), 81-93 [PMID: 6271633] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pPLaACR26 (LMBP 311) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Remaut et al., 1982. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.