Last data update: 24 January 2024 16:39 CET
Plasmid name: pREP4FLAG (LMBP 4343)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4343.gb
(View with Genome Compiler) p4343.txt p4343.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Epstein-Barr virus nuclear antigen 1 gene (EBNA1, EBNA-1, GeneID 3783709) FLAG epitope tag; N-terminal |
| Promoter: | Rous sarcoma virus long terminal repeat (RSV-LTR) Herpes simplex virus (HSV) thymidine kinase promoter (TK) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) |
| Selection marker: | Ampicillin (amp) Hygromycin (hyg) |
| Replicon: | Escherichia coli plasmid pMB1 origin Epstein-Barr virus origin of replication (oriP) |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pREP4 |
| Further information: | The plasmid was constructed by inserting two complementary oligonucleotides, containing a FLAG epitope and a BamHI site, between the filled-in BamHI and HindIII sites of pREP4 (Invitrogen). pREP4FLAG is an Epstein-Barr virus-based vector for episomal expression in mammalian cells. It contains the Rous sarcoma virus long terminal repeat for high-level expression of recombinant proteins. The expression cassette is provided with an N-terminal FLAG epitope tag, followed by a BamHI site. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727192.1. Other name of the plasmid is pREP4Flag. |
| EMBL Accession number: | LT727192.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 26/03/2001 |
| Sequence detail: | Sequence around the FLAG epitope tag:
RSV-LTR -> ------ FLAG epitope
5' ... TGTGCACCTCCAAGCTGGGTACCAGCTGCTAGCAAGCTACC.ATG.GAC.TAC.AAG.GAC.GAC
KpnI PvuII Met Asp Tyr Lys Asp Asp
NheI ***
---------->
GAT.GAC.AAG.AGG.ATC.CGA.TC ... 3'
Asp Asp Lys BamHI
***: start codon.
Punctuation indicates reading frame. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI and EcoRI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr L. Naumovski(1). (1) Division of Pediatric Hematology/Oncology, Stanford, USA |
| Plasmid reference: | Wong et al., Anal. Biochem. 252 (1997), 33-39 [PMID: 9324938] |
| Related plasmid reference: | Van Bogaert et al., J. Ind. Microbiol. Biotechnol. 35 (2008), 1085-1092 [PMID: 18594888] [DOI: 10.1007/s10295-008-0386-x] PhD thesis Van Bogaert et al. (2008) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pREP4FLAG (LMBP 4343) is available at BCCM/GeneCorner. This plasmid was deposited by Dr L. Naumovski and was published in Wong et al., 1997. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.