Last data update: 24 January 2024 16:39 CET
Plasmid name: pTRKH3 (LMBP 4462)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4462.gb
(View with Genome Compiler) p4462.txt p4462.pdf |
| Sequence analysis results Genecorner: |
|
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Tetracycline (tet) Erythromycin (ery) |
| Replicon: | Escherichia coli plasmid p15A origin Enterococcus faecalis plasmid pAMβ1 origin, incl. repD and repE |
| Copy number: | Medium copy number |
| Host range: | Escherichia coli Gram-positive bacterial strains |
| Parental clone: | pTRKH1 |
| Further information: | The plasmid was constructed by digesting the plasmid pTRKH1 with AvaI and religating, with loss of two small AvaI fragments. pTRKH3 is a shuttle cloning vector for E. coli, Lactococcus, Enterococcus, Streptococcus and Lactobacillus species. The vector has a medium copy number (30-40) in E. coli, and a high copy number (45-85) in streptococcal and lactococcal hosts. Tetracycline resistance is only expressed in E. coli, erythromycin resistance is expressed both in E. coli and in Gram-positive bacteria. The tetracycline resistance gene can be insertionally inactivated, facilitating screening for clones. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727213.1. |
| EMBL Accession number: | LT727213.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 15/12/2020 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: AvaI, EcoNI/KpnI, EcoRI/EcoRV and HindIII. This plasmid has also been fully sequenced. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr T.R. Klaenhammer(1). (1) North Carolina State University, USA |
| Plasmid reference: | O'Sullivan et al., Gene 137 (1993), 227-231 [PMID: 8299952] |
| Related plasmid reference: | PhD thesis Kassem et al. (2011) Papagianni et al., Anaerobe 30 (2014), 60-64 [PMID: 25199701] [DOI: 10.1016/j.anaerobe.2014.08.013] Brantl et al., Gene 142 (1994), 155-156 [PMID: 7514148] Sanders et al., J. Dairy Sci. 73 (1990), 2044-2053 [DOI: 10.3168/jds.S0022-0302(90)78883-2] Simon et al., Biochimie 70 (1988), 559-566 [PMID: 2844302] Dao et al., Appl. Environ. Microbiol. 49 (1985), 115-119 [PMID: 3883896] Behnke et al., Mol. Gen. Genet. 184 (1981), 115-120 [PMID: 6278245] Behnke et al., Mol. Gen. Genet. 182 (1981), 414-421 [PMID: 6272061] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 JM110 |
| Host reference: | Yanisch-Perron et al., Gene 33 (1985), 103-119 [PMID: 2985470] |
| Cultivation medium: | BHI + erythromycin (150 μg/ml) + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | The use of BHI medium (Brain Heart Infusion) instead of LB medium allows for the clear selection of erythromycin-resistant transformants of E. coli, using an antibiotic concentration of 150 μg/ml. |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pTRKH3 (LMBP 4462) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr T.R. Klaenhammer and was published in O'Sullivan et al., 1993. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.