Last data update: 24 January 2024 16:39 CET
Plasmid name: YCpCaMET15 (LMBP 4214)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4214.gb
(View with Genome Compiler) p4214.txt p4214.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Candida albicans bifunctional cysteine synthase/O-acetylhomoserine aminocarboxypropyltransferase genomic DNA (MET15, GeneID 3645031) |
| Promoter: | Candida albicans bifunctional cysteine synthase/O-acetylhomoserine aminocarboxypropyltransferase promoter (MET15) |
| Ribosome binding site: |
- |
| Terminator: | Candida albicans O-acetylhomoserine O-acetylserine sulfhydrylase terminator (CaMET15) |
| Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae URA3; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae centromeric region 4 (CEN4) Saccharomyces cerevisiae autonomously replicating sequence (ARS1) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-) |
| Parental clone: | YCp50 |
| Further information: | The plasmid was constructed as follows: the Sau3AI fragment of genomic DNA of C. albicans B2630, containing the MET15 gene, was inserted into the BamHI site of YCp50. To shorten the genomic DNA fragment, the plasmid was digested with EcoRI and the largest fragment was religated, eliminating also 377 bp of YCp50. The nucleotide sequence of the CaMET15 genomic DNA corresponds with the EMBL Nucleotide Sequence Database accession number AF188275.1. Referring to Viaene et al. (2000), sequence analysis of the genomic CaMET15 insert at the Department of Biomedical Molecular Biology (Ghent University, Belgium) revealed the presence of 1320 bp coding sequence, 542 bp upstream and 314 bp downstream sequence. According to the EMBL record AF188275 and the Candida genome (http://www.candidagenome.org/) however, 315 bp downstream sequence (incl. termination codon) is present. |
| EMBL Accession number: | AF188275.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 03/08/2001 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindII and NcoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Contreras(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Viaene et al., Yeast 16 (2000), 1205-1215 [PMID: 10992284] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
YCpCaMET15 (LMBP 4214) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Contreras and was published in Viaene et al., 2000. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.