Last data update: 24 January 2024 16:39 CET
Plasmid name: p55UASGLuc (LMBP 5279)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p5279.gb |
| Sequence analysis results Genecorner: |
NGS: e04-gc-may2021.fasta |
| Cloned DNA: |
Photinus pyralis (firefly) luciferase gene (LUC) |
| Promoter: | Saccharomyces cerevisiae GAL4 upstream activator element; synthetic sequence Phage T3 promoter Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | - |
| Parental clone: | p-55A2Luc |
| Further information: | The plasmid was constructed by substituting a SalI-BamHI fragment of p-55A2Luc, containing the kB motif, with the corresponding upstream activation sequence (UAS) element of the S. cerevisiae Gal4 promoter. This plasmid is useful for reporter gene assays to evaluate the transactivation potential of Gal4-fused transcription factors. |
| EMBL Accession number: | AC073072, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 09/06/2006 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr E. Aksoy(1). (1) William Harvey Research Institute, Queen Mary University of London, London, United Kingdom |
| Plasmid reference: | Yoneyama et al., EMBO J. 17 (1998), 1087-1095 [PMID: 9463386] |
| Related plasmid reference: | Yoneyama et al., J. Biochem. 120 (1996), 160-169 [PMID: 8864859] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
p55UASGLuc (LMBP 5279) is available at BCCM/GeneCorner. This plasmid was deposited by Dr E. Aksoy and was published in Yoneyama et al., 1998. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.