Last data update: 24 January 2024 16:39 CET
Plasmid name: pBAD33-Prc (LMBP 12415)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli tail-specific protease cDNA (prc, GeneID 946096) Escherichia coli DNA-binding transcriptional dual regulator AraC cDNA (araC, GeneID 944780) |
| Promoter: | Escherichia coli L-arabinose operon promoter (araB, araBAD, BAD) |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Chloramphenicol (cam) |
| Replicon: | Escherichia coli plasmid p15A origin |
| Copy number: | Low copy number |
| Host range: | Escherichia coli |
| Parental clone: | pBAD33 |
| Further information: | The plasmid was constructed by cloning the E. coli Prc coding sequence into the pBAD33 vector. Other name of the plasmid is pCK3. |
| EMBL Accession number: | - |
| Latest sequence update: | 04/03/2021 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr D.E. Culham(1) and Prof. Em. Dr J.M. Wood(1). (1) Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada |
| Plasmid reference: | Kerr et al., J. Bacteriol. 196 (2014), 1286-1296 [PMID: 24443528] [DOI: 10.1128/JB.00827-13] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 WG350 (LMBP 12428) |
| Host reference: | Culham et al., J. Mol. Biol. 229 (1993), 268-276 [PMID: 8421314] [DOI: 10.1006/jmbi.1993.1030] |
| Cultivation medium: | LB-Lennox + chloramphenicol (34 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pBAD33-Prc (LMBP 12415) is available at BCCM/GeneCorner. This plasmid was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Kerr et al., 2014. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.