Last data update: 24 January 2024 16:39 CET
Plasmid name: pGW8-mRoquin1-P2A-mCherry (LMBP 10833)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
|
| Cloned DNA: |
Mouse RING CCCH domains 1 cDNA (Rc3h1, Roquin-1, Roquin1, GeneID 381305) Porcine teschovirus-1 2A peptide (P2A) Discosoma sp. red fluorescent protein DNA (DsRed1); mutated monomeric variant (mCherry) |
| Promoter: | Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Streptomycin (Sm; spectinomycin (Sp)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Further information: | Gateway entry clone |
| EMBL Accession number: | - |
| Latest sequence update: | 07/04/2023 |
| Authenticity test: | The plasmid has been fully sequenced but the NGS data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr V. Heissmeyer(1). (1) Research Unit Molecular Immune Regulation, Helmholtz Zentrum München, Germany |
| Plasmid reference: | Jeltsch et al., Nat. Immunol. 15 (2014), 1079-1089 [PMID: 25282160] [DOI: 10.1038/ni.3008] |
| Related plasmid reference: | Vinuesa et al., Nature 435 (2005), 452-458 [PMID: 15917799] [DOI: 10.1038/nature03555] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + spectinomycin (150 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGW8-mRoquin1-P2A-mCherry (LMBP 10833) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr V. Heissmeyer and was published in Jeltsch et al., 2014. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.