Last data update: 24 January 2024 16:39 CET
Plasmid name: pHA-Ikkgamma (LMBP 4293)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human inhibitor of nuclear factor kappa B kinase regulatory subunit gamma cDNA (IKBKG, IP2, IP1, IKK-gamma, NEMO, FIP-3, FIP3, ZC2HC9, IKKG, GeneID 8517) |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pM |
| Further information: | The plasmid was constructed by cloning the human IKBKG coding sequence into the pM vector, after replacing the Gal4 DNA-binding domain by an HA tag. The nucleotide sequence of the human IKBKG coding sequence corresponds with the EMBL accession number AJ271718.1. Other name of the plasmid are pM-IKKγ and pHA-Ikkγ. |
| EMBL Accession number: | AJ271718.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 13/03/2023 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was obtained from Prof. Dr K.-T. Jeang(3). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium (3) Molecular Virology Section, Laboratory of Molecular Microbiology, National Institutes of Health, Bethesda, USA |
| Plasmid reference: | Jin et al., J. Biol. Chem. 274 (1999), 17402-17405 [PMID: 10364167] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pHA-Ikkgamma (LMBP 4293) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and was published in Jin et al., 1999. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.