Last data update: 24 January 2024 16:39 CET
Plasmid name: pHKG3 (LMBP 5036)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd) |
Promoter: | Simian virus 40 early promoter (SV40 early) |
Ribosome binding site: |
- |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Phage f1 origin |
Host range: | Escherichia coli Mammalian cells |
Further information: | - |
EMBL Accession number: | - |
Latest sequence update: | 31/07/2013 |
Sequence detail: | Sequence of the multi-cloning site: GALbd-> +++ +++ +++ 5' …TCG.CCGGAATTCGAGCTCTAGATAAGTAATGATCA… 3' EcoRI SacI BclI XbaI +++: termination codon. |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr T. Kouzarides(1). (1) The Gurdon Institute, Cambridge, United Kingdom |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pHKG3 (LMBP 5036) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr T. Kouzarides. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.