Last data update: 24 January 2024 16:39 CET
Plasmid name: pHLSec-SUMO-hDRD2-Avi-His (LMBP 10426)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10426.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human dopamine receptor D2 cDNA (DRD2, GeneID 1813) Gallus gallus protein tyrosine phosphatase receptor type S cDNA (PTPRS, R-PTP-S, CRYPALPHA, GeneID 396375); signal sequence, N-terminal Small ubiquitin-related modifier tag (SUMO tag); variant SUMOstar tag, N-terminal Avidin biotinylation sequence (AviTag); C-terminal Histidine tag (His-tag); C-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Chicken β-actin/rabbit β-globin hybrid promoter (AG) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pHLsec |
| Further information: | The plasmid was constructed by cloning the human DRD2 coding sequence, preceded by the PTPRS secretion signal and the SUMOstar tag and followed by an AviTag, into the pHLsec vector. The N and C-terminal tags can be removed from the encoded protein via a CASP3 cleavage site. The SUMOstar tag carries the mutations R64T and R71E (Liu et al., 2008). Other name of the plasmid is pHLSec-SUMO-D2-Avi-His. |
| EMBL Accession number: | - |
| Latest sequence update: | 16/08/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr S. Savvides(1)(2). It was constructed by Dr. I. Ramou(1)(2). (1) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium (2) VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Aricescu et al., Acta Crystallogr. D Biol. Crystallogr. 62 (2006), 1243-1250 [PMID: 17001101] [DOI: 10.1107/S0907444906029799] Liu et al., Protein Expr. Purif. 62 (2008), 21-28 [PMID: 18713650] [DOI: 10.1016/j.pep.2008.07.010] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pHLSec-SUMO-hDRD2-Avi-His (LMBP 10426) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr S. Savvides. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.