Last data update: 24 January 2024 16:39 CET
Plasmid name: pHSG272 (LMBP 1106)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | phsg272.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Herpes simplex virus (HSV) thymidine kinase promoter (TK) pBR322 P1 promoter |
| Ribosome binding site: |
- |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) |
| Selection marker: | Neomycin (neo; G418; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid ColE1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pHSG262; pAG60 |
| Further information: | The cosmid contains a unique BamHI site, allowing cloning of Sau3AI, MboI, BglII, BclI and BamHI generated fragments. pHSG272 contains the Tn5 kanamycin resistance (neo) gene which is selectable in both prokaryotic and mammalian cells, making this cosmid suitable for transferring DNA into mammalian cells. The nucleotide sequence has been compiled in accordance with the description in Brady et al. (1984), Colbère-Garapin et al. (1979) and Colbère-Garapin et al. (1981), but contains some uncertainties. |
| EMBL Accession number: | - |
| Latest sequence update: | 14/01/1997 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr G. Schütz(1). (1) German Cancer Research Center, Heidelberg, Germany |
| Plasmid reference: | Brady et al., Gene 27 (1984), 223-232 [PMID: 6327467] |
| Related plasmid reference: | Colbère-Garapin et al., J. Mol. Biol. 150 (1981), 1-14 [PMID: 6271971] [DOI: 10.1016/0022-2836(81)90321-1] Colbère-Garapin et al., Proc. Natl. Acad. Sci. U.S.A. 76 (1979), 3755-3759 [PMID: 226978] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061(λ) |
| Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
| Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pHSG272 (LMBP 1106) is available at BCCM/GeneCorner. This plasmid was deposited by Dr G. Schütz and was published in Brady et al., 1984. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.