Last data update: 24 January 2024 16:39 CET
Plasmid name: pHT304-18Z-cytK-CFPopt (LMBP 7641)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p7641.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Synthetic cyan fluorescent protein DNA (CFP); low GC optimised (CFPopt) B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene) Escherichia coli lac Z gene (lacZ) |
| Promoter: | Bacillus cereus cytotoxin K promoter (cytK) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Erythromycin (ery) |
| Replicon: | Escherichia coli plasmid pMB1 origin Bacillus thuringiensis plasmid pHT1030 origin |
| Host range: | Escherichia coli; use a ccdB-resistant strain for propagation Bacillus sp. |
| Parental clone: | pHT304-18Z |
| Further information: | The plasmid was created by inserting the cytK promoter, obtained from B. cereus ATCC 14579 genomic DNA, and the CFPopt gene, obtained from the pSW4-CFPopt plasmid (Sastalla et al. 2009), in the pHT304-18Z plasmid (Agaisse & Lereclus 1994). The plasmid-carrying strain was an intermediate in the construction of the B. cereus reporter strain for CytK toxin expression. This plasmid is also available in the host strain "Bacillus cereus Frankland and Frankland 1887 AL" under accession number LMBP 07642. Other name of the plasmid is pHT304-CytK-CFPopt. |
| EMBL Accession number: | - |
| Latest sequence update: | 26/03/2012 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI, EcoRV, HincII and PvuI. As compared to the compiled nucleotide sequence, there is an extra HincII site in the lacZ cds. The EcoRV site at position 927 is located around positoin 1250. The EcoRI site at position 1372 could not be experimentally confirmed. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by S. Ceuppens(1) and Prof. Dr N. Boon (2). It was constructed by S. Ceuppens(1). (1) Department of Food Safety and Food Quality, Ghent University, Ghent, Belgium (2) Department of Biochemical and Microbial Technology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Ceuppens et al., J. Appl. Microbiol. 114 (2013), 899-906 [PMID: 23216529] [DOI: 10.1111/jam.12096] Agaisse et al., Mol. Microbiol. 13 (1994), 97-107 [PMID: 7984098] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 TOP10 |
| Host reference: | Instruction Manual (Invitrogen) |
| Related host reference: | Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | LMBP 07642 |
Refer in your Materials and Methods: |
pHT304-18Z-cytK-CFPopt (LMBP 7641) is available at BCCM/GeneCorner. This plasmid was deposited by S. Ceuppens and Prof. Dr N. Boon . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.