Last data update: 24 January 2024 16:39 CET
Plasmid name: pHV33 (LMBP 8033)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Chloramphenicol (cam) Tetracycline (tet) |
| Replicon: | - |
| Host range: | Escherichia coli |
| Parental clone: | - |
| Further information: | The plasmid is a hybrid between pC194 and pBR322 by fusion on the HindIII sites. The vector is meant for heterospecific cloning. |
| EMBL Accession number: | - |
| Latest sequence update: | 18/03/2013 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by M. Mergeay(1) and A. Provoost(1). (1) SCK.CEN, Mol, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Goze and Ehrlich, Proc. Natl. Acad. Sci. U.S.A. 77 (1980), 7333-7337 [PMID: 7012836] [DOI: 10.1073/pnas.77.12.7333] Primrose and Ehrlich, Plasmid 6 (1981), 193-201 [PMID: 7029580] [DOI: 10.1016/0147-619x(81)90066-4] Bolivar et al., Gene 2 (1977), 95-113 [PMID: 344137] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MM294 |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (34 μg/ml) + tetracycline (10 μg/ml)* |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin and tetracycline. |
| Other culture collection numbers: | SCK CM0278 |
Refer in your Materials and Methods: |
pHV33 (LMBP 8033) is available at BCCM/GeneCorner. This plasmid was deposited by M. Mergeay and A. Provoost. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.