Last data update: 24 January 2024 16:39 CET
Plasmid name: pLA-Flag-Cullin3 (LMBP 11168)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p11168.gb |
| Sequence analysis results Genecorner: |
NGS: g03-gc-dec2018.fasta |
| Cloned DNA: |
Human cullin 3 cDNA (CUL3, CUL-3, PHA2E, GeneID 8452) FLAG epitope tag; N-terminal |
| Promoter: | Rous sarcoma virus/human immunodeficiency virus hybrid long terminal repeat (RSV/HIV-1 5' LTR) Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rous sarcoma virus/human immunodeficiency virus hybrid long terminal repeat (RSV/HIV-1 3' LTR); modified HIV-1 3' LTR with deleted U3 region (ΔU3/3' LTR) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pLA |
| Further information: | The plasmid was constructed by cloning the FLAG-tagged human CUL3 coding sequence into the pLA vector. Because of the high risk for recombination, this plasmid is only available under the format of isolated plasmid DNA. Other name of the plasmid is pLA Flag Cullin3 (1467). |
| EMBL Accession number: | - |
| Latest sequence update: | 24/01/2019 |
| Authenticity test: | This plasmid has been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr Anna Sablina(1)(2). (1) Center for Cancer Biology, VIB, Leuven, Belgium (2) Department of Oncology, University of Leuven, Leuven, Belgium |
| Plasmid reference: | Steklov et al., Science 362 (2018), 1177-1182 [PMID: 30442762] [DOI: 10.1126/science.aap7607] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 in E. coli; L2 in mammalian cells |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLA-Flag-Cullin3 (LMBP 11168) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr Anna Sablina and was published in Steklov et al., 2018. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.