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GeneCorner plasmid details

Last data update: 21 October 2021 10:13 CEST

Plasmid name: pMG1-MAPPIT (LMBP 7067)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: -
Sequence
analysis results
Genecorner:

-

Cloned DNA: Human interleukin 6 cytokine family signal transducer cDNA (IL6ST, GP130, CD130, sGP130, IL-6RB, GeneID 3572); fragment
B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)
Xpress epitope; N-terminal
Histidine tag (His-tag); N-terminal
FLAG epitope tag; N-terminal
Promoter: Simian virus 40 early/human T-cell lymphotropic virus type 1 LTR hybrid promoter (SRα)
Escherichia coli lac operon promoter; mutant (lacUV5)
Ribosome
binding site:
-
Terminator: Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Chloramphenicol (cam)
Replicon: Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli
Mammalian cells; SV40 permissive cells
Parental clone: pMG1
Further information: The plasmid was constructed by cloning a Gateway cassette into the pMG1 vector.
This Gateway destination vector allows the construction of prey plasmids for Mammalian Protein-Protein Interaction Trap (MAPPIT) analysis. It is compatible with the human orfeome library which is also available at BCCM/GeneCorner.
Other name of the plasmid is pMG1-Gateway.
EMBL Accession number: -
Latest sequence update: 22/03/2011
Authenticity test: The plasmid still needs to be subjected to the authenticity test.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr F. Van Roy(1) (2). It was constructed by I. Kahr(1)(2).
(1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pMG1-MAPPIT (LMBP 7067) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr F. Van Roy .

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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