Last data update: 21 October 2021 10:13 CEST
Plasmid name: pMG1-MAPPIT (LMBP 7067)
|New search||Print data sheet|
|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner non-core plasmid|
Human interleukin 6 cytokine family signal transducer cDNA (IL6ST, GP130, CD130, sGP130, IL-6RB, GeneID 3572); fragment|
B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)
Xpress epitope; N-terminal
Histidine tag (His-tag); N-terminal
FLAG epitope tag; N-terminal
|Promoter:||Simian virus 40 early/human T-cell lymphotropic virus type 1 LTR hybrid promoter (SRα)
Escherichia coli lac operon promoter; mutant (lacUV5)
|Terminator:||Simian virus 40 polyadenylation signal (SV40 polyA)|
|Selection marker:||Ampicillin (amp)
|Replicon:||Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli
Mammalian cells; SV40 permissive cells
|Further information:||The plasmid was constructed by cloning a Gateway cassette into the pMG1 vector.
This Gateway destination vector allows the construction of prey plasmids for Mammalian Protein-Protein Interaction Trap (MAPPIT) analysis. It is compatible with the human orfeome library which is also available at BCCM/GeneCorner.
Other name of the plasmid is pMG1-Gateway.
|EMBL Accession number:||-|
|Latest sequence update:||22/03/2011|
|Authenticity test:||The plasmid still needs to be subjected to the authenticity test.|
|History of deposit:||This plasmid was deposited by Prof. Dr F. Van Roy(1) (2). It was constructed by I. Kahr(1)(2).
(1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium
(2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pMG1-MAPPIT (LMBP 7067) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr F. Van Roy .|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.