Last data update: 24 January 2024 16:39 CET
Plasmid name: pMaT4HTNFm131Q (LMBP 2642)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p2642.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human tumor necrosis factor cDNA (TNF); mutated mature sequence |
Promoter: | Phage T4 gene 32 promoter (T4g32) |
Ribosome binding site: |
Ribosome binding site (RBS) of the phage T4 gene 32 (T4g32) |
Terminator: | Phage fd terminator Escherichia coli tryptophan operon terminator (trp); synthetic sequence |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
Host range: | Escherichia coli |
Parental clone: | pMa-T4-hTNF-TA |
Further information: | The plasmid was constructed by site-specific mutagenesis, where the nucleotide sequence of AA131 (CGA, coding for Arg) was converted to CAG (coding for Gln), creating a unique EspI site. After mutagenesis, use mutS strains for primary transformation (e.g. sup(-) strain WK6mutS, sup(+) strain BMH71-18mutS); for segregation of possible mutants: sup(-) strains (e.g. WK6). The nucleotide sequence of the wt hTNF cDNA corresponds with the EMBL Nucleotide Sequence Database accession number X01394.1. |
EMBL Accession number: | X01394.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 21/01/1993 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | Tavernier et al., J. Mol. Biol. 211 (1990), 493-501 [PMID: 1689779] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 SURE |
Host reference: | Greener, Strategies 3 (1990), 5-6 |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pMaT4HTNFm131Q (LMBP 2642) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and was published in Tavernier et al., 1990. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.