Last data update: 24 January 2024 16:39 CET
Plasmid name: pROSA26-DV1-mCtnna1 (LMBP 9405)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse catenin (cadherin associated protein), alpha 1 cDNA (Ctnna1, Catna1, GeneID 12385) Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP) Mouse gene trap ROSA 26 gene (Gtrosa26, Gt(ROSA)26Sor, beta geo, Gtrgeo26, R26, ROSA26, Thumpd3as1, GeneID 14910); 5' UTR and 3' UTR |
| Promoter: | Mouse phosphoglycerate kinase 1 promoter (PGK1) Phage T3 promoter Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter |
| Ribosome binding site: |
Internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) polyprotein gene |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) Bovine growth hormone polyadenylation signal (BGH polyA) Mouse phosphoglycerate kinase 1 polyadenylation signal (PGK1 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pROSA26-DV1 |
| Further information: | The plasmid was constructed by cloning the mouse Ctnna1 coding sequence into the pROSA26-DV1 vector via Gateway LR recombination. The endogeneous ROSA26 promoter is used to drive transgene expression in mouse ES cells. The G4 ROSALUC ES cells (LMBP 10507CB) are available at BCCM/GeneCorner as well. The nucleotide sequence of the parental pROSA26-DV1 plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726830.1. Other name of the plasmid is pRosaDV-maEctncDNA. |
| EMBL Accession number: | LT726830.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 19/04/2021 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr F. Van Roy(1) (2). It was constructed by K. Tyberghein(1)(2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Nyabi et al., Nucleic Acids Res. 37 (2009), e55 [PMID: 19279185] [DOI: 10.1093/nar/gkp112] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli Electromax STBL4 |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pROSA26-DV1-mCtnna1 (LMBP 9405) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr F. Van Roy . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.