Last data update: 24 January 2024 16:39 CET
Plasmid name: pRPneo (LMBP 4787)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner core plasmid |
GeneCorner sequence: |
p4787.gb
(View with Genome Compiler) p4787.txt p4787.pdf |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
BK virus DNA (BKV); fragment of the Dunlop strain |
Promoter: | Simian virus 40 early promoter (SV40 early) BK virus early promoter (BKV early) BK virus late promoter (BKV late) |
Ribosome binding site: |
- |
Terminator: | BK virus polyadenylation signal (BKV polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) Neomycin (neo; G418; kanamycin (kan)) |
Replicon: | Escherichia coli plasmid pMB1 origin BK virus origin (BKV) Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pRP; pSV2Neo |
Further information: | The plasmid was constructed as follows: the NdeI-BamHI fragment from pSV2Neo, containing the SV40 early promoter, the neomycin resistance gene and the SV40 polyA signal, was provided with BamHI linkers and inserted into the unique BamHI site of pRP. The orientation of the inserted fragment was experimentally verified. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727035.1.1 Other name of the plasmid is pRP-neo. |
EMBL Accession number: | LT727035.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 10/06/1994 |
Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI, HindII and SphI. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr G. Barbanti-Brodano(1). (1) Institute of Microbiology, School of Medicine, University of Ferrara, Italy |
Plasmid reference: | Grossi et al., Arch. Virol. 99 (1988), 249-259 [PMID: 2835949] |
Related plasmid reference: | Van Craenenbroeck et al., Gene 253 (2000), 293-301 [PMID: 10940567] Seif et al., Cell 18 (1979), 963-977 [PMID: 229976] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pRPneo (LMBP 4787) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Barbanti-Brodano and was published in Grossi et al., 1988. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.