Last data update: 24 January 2024 16:39 CET
Plasmid name: pRSF1b-GspE-AVI (LMBP 11263)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | - |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Pseudomonas aeruginosa type II general secretion pathway protein E cDNA (GspE, xcpR, GeneID 877804) Histidine tag (His-tag); N-terminal Avidin biotinylation sequence (AviTag); C-terminal Escherichia coli lac repressor gene (lacI) |
Promoter: | Phage T7 gene 10 promoter (T7g10) Escherichia coli β-lactamase promoter (amp) |
Ribosome binding site: |
- |
Terminator: | Phage T7 gene 10 terminator (T7g10) |
Selection marker: | Neomycin (neo; kanamycin (kan)) |
Replicon: | Broad-host-range plasmid RSF1030 origin of replication |
Host range: | Escherichia coli |
Parental clone: | pRSF-1b |
Further information: | The plasmid was constructed by cloning the P. aeruginosa GspE (UNIPROT code: Q0512) coding sequence into the KpnI/HindIII opened pRSF-1b vector. |
EMBL Accession number: | - |
Latest sequence update: | 27/06/2019 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | The plasmid was constructed by A. Fulara(1)(2) and Prof. Dr S. Savvides(1)(2). 1) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium (2) VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium |
Plasmid reference: | Fulara et al., bioRxiv (2020), 1-20 [DOI: 10.1101/2020.03.20.999888] |
Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli B BL21 |
Host reference: | - |
Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pRSF1b-GspE-AVI (LMBP 11263) is available at BCCM/GeneCorner. The plasmid was constructed by A. Fulara and Prof. Dr S. Savvides and was published in Fulara et al., 2020. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.