Last data update: 24 January 2024 16:39 CET
Plasmid name: pSC200 (LMBP 10349)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10349.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli HTH-type transcriptional activator cDNA (rhaR, rhaC1) Escherichia coli HTH-type transcriptional activator cDNA (rhaS, rhaC2) |
| Promoter: | Escherichia coli L-rhamnulokinase promoter (rhaB) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Trimethoprim (tmp) |
| Replicon: | Broad-host-range plasmid RK2/RP4 origin of transfer (oriT) Escherichia coli plasmid R6K origin; defective pir gene |
| Host range: | Escherichia coli; use strains supplying the R6K pir function Gram-negative bacterial strains |
| Parental clone: | pGP704 |
| Further information: | The plasmid was constructed by cloning the rhamnose-inducible rhaB promoter cassette into the pGP704 vector. The plasmid can be used for the construction of conditional mutants of Gram-negative bacteria. |
| EMBL Accession number: | - |
| Latest sequence update: | 08/05/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr A. Sass(1). It was obtained from Prof. Dr M. Valvano(2). (1) Laboratory of Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University, Ghent, Belgium (2) Infectious Diseases Research Group, Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada |
| Plasmid reference: | Ortega et al., J. Bacteriol. 189 (2007), 3639-3644 [PMID: 17337576] [DOI: 10.1128/JB.00153-07] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 S17-1(λpir) |
| Host reference: | Tascon et al., J. Bacteriol. 175 (1993), 5717-5722 [PMID: 8396122] [DOI: 10.1128/jb.175.17.5717-5722.1993] |
| Related host reference: | Kalogeraki et al., Gene 188 (1997), 69-75 [PMID: 9099861] Simon et al., Biotechnology 1 (1983), 784-791 [DOI: 10.1038/nbt1183-784] |
| Cultivation medium: | LB-Lennox + trimethoprim (50 µg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSC200 (LMBP 10349) is available at BCCM/GeneCorner. The plasmid was deposited by Dr A. Sass and was published in Ortega et al., 2007. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.