Last data update: 24 January 2024 16:39 CET
Plasmid name: pSCrhaB2 (LMBP 10354)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p10354.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Escherichia coli HTH-type transcriptional activator cDNA (rhaR, rhaC1) Escherichia coli HTH-type transcriptional activator cDNA (rhaS, rhaC2) |
Promoter: | Escherichia coli L-rhamnulokinase promoter (rhaB) |
Ribosome binding site: |
- |
Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
Selection marker: | Trimethoprim (tmp) |
Replicon: | Broad-host-range Gram-negative Bordetella bronchiseptica S87 plasmid pBBR1 replicon |
Host range: | Escherichia coli Gram-negative bacterial strains |
Parental clone: | pMLBAD |
Further information: | The plasmid was constructed by removing the araC gene and the BAD promoter from pMLBAD via NsiI/NcoI restriction digest, and replacing it with the rhamnose inducible promoter cassette. This plasmid is meant for protein expression in Gram negative bacteria, with a rhamnose-inducible rhaB promoter and transcriptional regulation genes rhaS and rhaR from E.coli. The nucleotide sequence of the pSCrhaB2 vector corresponds with the EMBL Nucleotide Sequence Database accession number AY917125.1. |
EMBL Accession number: | AY917125.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 05/05/2017 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | The plasmid was deposited by Dr A. Sass(1). It was obtained from Prof. Dr M. Valvano(2). (1) Laboratory of Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University, Ghent, Belgium (2) Infectious Diseases Research Group, Department of Microbiology and Immunology, University of Western Ontario, London, Ontario, Canada |
Plasmid reference: | Cardona et al., Plasmid 54 (2005), 219-228 [PMID: 15925406] [DOI: 10.1016/j.plasmid.2005.03.004] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + trimethoprim (50 µg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSCrhaB2 (LMBP 10354) is available at BCCM/GeneCorner. The plasmid was deposited by Dr A. Sass and was published in Cardona et al., 2005. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.