Last data update: 24 January 2024 16:39 CET
Plasmid name: pSCrhaM2-mCherry (LMBP 10357)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10357.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Discosoma sp. red fluorescent protein DNA (DsRed1); mutated monomeric variant (mCherry) Escherichia coli HTH-type transcriptional activator cDNA (rhaR, rhaC1) Escherichia coli HTH-type transcriptional activator cDNA (rhaS, rhaC2) |
| Promoter: | Escherichia coli L-rhamnulokinase promoter (rhaB) |
| Ribosome binding site: |
- |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Trimethoprim (tmp) |
| Replicon: | Broad-host-range Gram-negative Bordetella bronchiseptica S87 plasmid pBBR1 replicon |
| Host range: | Escherichia coli Gram-negative bacterial strains |
| Parental clone: | pSCrhaM2 |
| Further information: | The plasmid was constructed by cloning the mCherry reporter gene into the XbaI/HindIII opened pSCrhaM2 vector. This plasmid is an RNA expression vector for Gram negative bacteria, with the rhamnose-inducible rhaB promoter and transcriptional regulation genes rhaS and rhaR from E.coli. The plasmid can be used for translational fusions by placing native 5’ UTRs under control of the rhaB promoter with mCherry as reporter. The nucleotide sequence of the parental pSCrhaB2 vector corresponds with the EMBL Nucleotide Sequence Database accession number AY917125.1. |
| EMBL Accession number: | AY917125.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 05/05/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr A. Sass(1). (1) Laboratory of Pharmaceutical Microbiology, Department of Pharmaceutical Analysis, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Cardona et al., Plasmid 54 (2005), 219-228 [PMID: 15925406] [DOI: 10.1016/j.plasmid.2005.03.004] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + trimethoprim (50 µg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSCrhaM2-mCherry (LMBP 10357) is available at BCCM/GeneCorner. The plasmid was deposited by Dr A. Sass. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.