Last data update: 24 January 2024 16:39 CET
Plasmid name: pSE380igaAB (LMBP 3236)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p3236.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Neisseria gonorrhoeae NCTC8375 gene encoding IgA1 protease (iga); α and β domains (igaαβ) Escherichia coli lac repressor gene (lacI) |
| Promoter: | Escherichia coli hybrid tryptophan/lacUV5 promoter (trc) Escherichia coli lac repressor promoter; mutant (lacI(q)) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pSE380; pMc514igam2 |
| Further information: | The plasmid was constructed by inserting the HindIII-ScaI fragment from pMc514igam2, encoding the α and β domains of the Neisseria gonorrhoeae NCTC8375 IgA1 protease, into the HindIII-Eco47III opened pSE380 vector. pSE380igaAB is an intermediate plasmid in the construction strategy of the expression vector pQEB1sFvigaAB. To reconstruct the nucleotide sequence of this plasmid, the sequence of the IgA1 protease gene from Neisseria gonorrhoeae MS11 corresponds with the EMBL Nucleotide Sequence Database (Release 36; Accession number X04835). However, the cloned IgA1 protease gene fragment was derived from Neisseria gonorrhoeae NCTC8375 and may be different in several nucleotides. As such, there is uncertainty as to the cutting frequency of the restriction enzymes indicated. Other name of the plasmid is pSE380 IgAαβ. |
| EMBL Accession number: | - |
| Latest sequence update: | 19/06/1995 |
| Sequence detail: | Nucleotide sequence at the Eco47III/ScaI fusion.
---> pSE380 multilinker
Met Ala Gly Asp His Val Val Glu Cys Leu Arg Ile Gln
5' ACC ATG.GCT.GGT.GAC.CAC.GTC.GTG.GAA.TGC.CTT.CGA.ATT.CAG.
NcoI BstEII EcoRI
His Leu His Met Gly Arg Arg Pro Glu Val Ile Ile Thr Arg
CAC.CTG.CAC.ATG.GGA.CGT.CGA.CCT.GAG.GTA.ATT.ATA.ACC.CGG.
SalI SmaI
ApaI
Ala Leu Tyr Met Asp Pro Ile Ala Met Ile Ile Met Thr Asp
GCC.CTA.TAT.ATG.GAT.CCA.ATT.GCA.ATG.ATC.ATC.ATG.ACA.GAT.
BamHI BclI BglII
|---> alpha protein sequence
Leu Arg Ala Ile Asp Ile Ser|Thr Pro Ala Ala Asn Thr
CTG.CGC.GCG.ATC.GAT.ATC.AGC|ACT.CCT.GCC.GCA.AAC.ACG. 3'
ClaI |
EcoRV |
-------
Eco47III/ScaI fusion |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by I. Poelaert(1) (2) and Prof. Dr E. Remaut(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Irmgard Poelaert (1995) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 RR1 |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSE380igaAB (LMBP 3236) is available at BCCM/GeneCorner. This plasmid was deposited by I. Poelaert and Prof. Dr E. Remaut and was published in Irmgard Poelaert, 1995. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.