Last data update: 24 January 2024 16:39 CET
Plasmid name: pSG5-TIF2 (LMBP 4138)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p4138.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human transcription intermediary factor 2 cDNA (TIF2) |
Promoter: | Simian virus 40 early promoter (SV40 early) Phage T7 gene 10 promoter (T7g10) |
Ribosome binding site: |
- |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pSG5 |
Further information: | The plasmid was constructed by cloning the human TIF2 cDNA into the EcoRI-opened pSG5 vector. This is a high copy number plasmid. The highest level of expression is obtained in cells expressing the T antigen. The nucleotide sequence of the human TIF2 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number X97674.1. The nucleotide sequence of the pSG5 vector corresponds with the EMBL Nucleotide Sequence Database accession number AF013258.1. |
EMBL Accession number: | X97674.1, view at EMBL, GenBank, DDBJ AF013258.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 28/10/2013 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr P. Chambon(1). It was constructed by Dr J. Voegel(1). (1) Institut de Genetique et de Biologie Moleculaire et Cellulaire, Illkirch, Strasbourg, France |
Plasmid reference: | Voegel et al., EMBO J. 15 (1996), 3667-3675 [PMID: 8670870] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSG5-TIF2 (LMBP 4138) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Chambon and was published in Voegel et al., 1996. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.