Last data update: 17 September 2021 10:55 CEST
Plasmid name: pSP64Mx (LMBP 2164)
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|Price category:||Cat. 1 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Promoter:||Mouse Mx promoter
Escherichia coli lac operon promoter
Phage SP6 promoter
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin|
|Host range:||Escherichia coli|
|Further information:||The plasmid was constructed by inserting the PstI-PvuII fragment of the mouse Mx promoter sequence into the unique PstI and HindII sites of pSP64; this resulted in the loss of the unique HindII and the unique SalI site of the pSP64 polylinker.
This plasmid contains the mouse Mx promoter including 19 nucleotides downstream from the TATA-box, ending at position -1 which is the middle of the lost PvuII site.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727626.1.
|EMBL Accession number:||LT727626.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||07/02/1990|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BglI/EcoRI, DrdI, EcoRI, PvuI and SacI.|
|History of deposit:||This plasmid was deposited by Prof. Dr C. Weissmann(1).
(1) University Zurich, Switzerland
|Plasmid reference:||Hug et al., Mol. Cell. Biol. 8 (1988), 3065-3079 [PMID: 2974922]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Refer in your Materials and Methods:
|pSP64Mx (LMBP 2164) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr C. Weissmann and was published in Hug et al., 1988.|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.