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GeneCorner plasmid details

Last data update: 17 September 2021 10:55 CEST

Plasmid name: pSP64Mx (LMBP 2164)

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Price category: Cat. 1 (cf. price list)
Status: GeneCorner core plasmid
GeneCorner sequence: psp64mx.gb (View with Genome Compiler)
psp64mx.pdf
Sequence
analysis results
Genecorner:

-

Cloned DNA: -
Promoter: Mouse Mx promoter
Escherichia coli lac operon promoter
Phage SP6 promoter
Ribosome
binding site:
-
Terminator: -
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Host range: Escherichia coli
Parental clone: pSP64
Further information: The plasmid was constructed by inserting the PstI-PvuII fragment of the mouse Mx promoter sequence into the unique PstI and HindII sites of pSP64; this resulted in the loss of the unique HindII and the unique SalI site of the pSP64 polylinker.
This plasmid contains the mouse Mx promoter including 19 nucleotides downstream from the TATA-box, ending at position -1 which is the middle of the lost PvuII site.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727626.1.
EMBL Accession number: LT727626.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 07/02/1990
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: BglI/EcoRI, DrdI, EcoRI, PvuI and SacI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Prof. Dr C. Weissmann(1).
(1) University Zurich, Switzerland
Plasmid reference: Hug et al., Mol. Cell. Biol. 8 (1988), 3065-3079 [PMID: 2974922]
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 MC1061
Host reference: Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
Related host reference: Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Refer in your Materials and Methods:

pSP64Mx (LMBP 2164) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr C. Weissmann and was published in Hug et al., 1988.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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