Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV-Sport-HIF-Rluc (LMBP 5217)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5217.gb
(View with Genome Compiler) p5217.txt p5217.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Renilla reniformis luciferase gene (rLUC) |
| Promoter: | Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter; mutant (lacUV5) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
Internal ribosome entry site (IRES) of the mouse hypoxia-inducible factor-1 α subunit (HIF-1α) |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV SPORT 1; pCAGGS-HIF-Rluc |
| Further information: | The construction of the plasmid is described in Schepens et al. (2005). pSV-Sport-HIF-Rluc is a monocistronic expression vector with the internal ribosome entry site (IRES) of the mouse HIF-1α subunit upstream of the rLUC coding sequence. This plasmid is useful to demonstrate that the HIF-1α IRES could sustain luciferase expression in hypoxic conditions. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT726975.1. The nucleotide sequence of the mouse HIF-1α 5' UTR corresponds with the EMBL Nucleotide Sequence Database accession number Y13656.1. Name mentioned in Schepens et al. (2005) is pSV-HIF-Rluc. |
| EMBL Accession number: | Y13656.1, view at EMBL, GenBank, DDBJ LT726975.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 01/12/2006 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: Eco130I, EcoRI, HpaI and PstI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr B. Schepens(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Schepens et al., Nucleic Acids Res. 33 (2005), 6884-6894 [PMID: 16396835] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
| Related website: | http://www.iresite.org/IRESite_web.php?page=view&entry_id=1 |
Refer in your Materials and Methods: |
pSV-Sport-HIF-Rluc (LMBP 5217) is available at BCCM/GeneCorner. This plasmid was deposited by Dr B. Schepens and Prof. Dr R. Beyaert and was published in Schepens et al., 2005. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.