Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV-Sport-RF-EMCV (LMBP 5250)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p5250.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Renilla reniformis luciferase gene (rLUC) Photinus pyralis (firefly) luciferase gene (LUC); mutated coding region (LUCm; luc(+)) |
| Promoter: | Simian virus 40 early promoter (SV40 early) Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter; mutant (lacUV5) |
| Ribosome binding site: |
Internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) polyprotein gene |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSVSportlacZEMCVFluc; pSV-Sport-Rluc |
| Further information: | The plasmid was created by isolating the rLUC coding sequence from pSV-Sport-Rluc and cloning it into the pSVSportlacZEMCVFluc vector, replacing the lacZ gene. This plasmid is a dicistronic expression vector with the internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) in the intercistronic region between upstream rLUC and downstream LUCm coding sequences. The IRES can drive translation of the downstream LUCm sequence independently of the 5'-cap structure bound to the 5'-end of the mRNA molecule. Other names of the plasmid are pcDNA3RLucEMCVFLuc and pcDNA3-RF-EMCV. |
| EMBL Accession number: | - |
| Latest sequence update: | 28/04/2016 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HaeII, HpaI, KpnI/XbaI, NcoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Y. Bruynooghe(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Sherf et al., Promega Notes Magazine 49 (1994), 14-21 Tinton et al., Biochem. J. 385 (2005), 155-163 [PMID: 15330758] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV-Sport-RF-EMCV (LMBP 5250) is available at BCCM/GeneCorner. This plasmid was deposited by Y. Bruynooghe and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.