Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV2CatR (LMBP 8037)
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Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
- |
Promoter: | Simian virus 40 early promoter (SV40 early) |
Ribosome binding site: |
- |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) Chloramphenicol (cam) |
Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pBR322::Tn9; pSV2 |
Further information: | The plasmid was created by cloning a Tag1 fragment of pBR322::Tn9 into pSV2. |
EMBL Accession number: | - |
Latest sequence update: | 18/03/2013 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by M. Mergeay(1) and A. Provoost(1). (1) SCK.CEN, Mol, Belgium |
Plasmid reference: | - |
Related plasmid reference: | Zhang et al., Mol. Cell. Biol. 24 (2004), 1044-1057 [PMID: 14729952] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 N6302[pCW2] |
Host reference: | - |
Helper plasmid: | pCW2 |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + chloramphenicol (34 μg/ml)* |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with ampicillin. |
Other culture collection numbers: | SCK CM0287 |
Refer in your Materials and Methods: |
pSV2CatR (LMBP 8037) is available at BCCM/GeneCorner. This plasmid was deposited by M. Mergeay and A. Provoost. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.