Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV51E6Hy3f22 (LMBP 1983)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p1983.gb
(View with Genome Compiler) p1983.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse anti-hPLAP monoclonal antibody E6(Hy3,κ) cDNA; truncated mouse::human chimeric heavy chain of the F(ab')2 fragment E6(Hy3,κ)F2 (E6Hy3f2) |
| Promoter: | Simian virus 40 early promoter (SV40 early) Simian virus 40 late promoter (SV40 late) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV51; pBMGNeoE6Hy3f2 |
| Further information: | The plasmid was constructed by inserting the E6Hy3f2 gene as a SalI fragment from pBMGNeoE6Hy3f2 into the SalI vector fragment of pSV51 (nucleotide positions 3095 and 3628). This plasmid is very similar to pSV51E6Hy3f21, but it was made in another way. The truncated CH2 domain consists of 31 amino acids, terminating at the multi-stop-linker. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727612.1. Other name of the plasmid is pSV51E6Hy3f2. |
| EMBL Accession number: | LT727612.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 05/09/1990 |
| Sequence detail: | Terminal sequence of the truncated CH2 domain:
CH2 pSV51
-------------- --------->
Arg Thr Pro Asp Leu Val Asn *
5' CGG ACC CCT GAC TTA GTT AAC TAA GCT CGTC GACCCTCTAG 3'
^ HpaI Filled- SalI
in
HindIII
-------------------
Linker
^: 27th amino acid of CH2.
*: Termination codon. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI/XmnI, EcoRI, EcoRV, PvuI/XbaI, PvuII and SalI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by V. Feys(1) and Prof. Dr W. Fiers(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12xB HB101 |
| Host reference: | Boyer and Roulland-Dussoix, J. Mol. Biol. 41 (1969), 459-472 [PMID: 4896022] |
| Related host reference: | Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Sambrook et al. (eds), Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, NY (1989) [ISSN/ISBN: 0879693096] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV51E6Hy3f22 (LMBP 1983) is available at BCCM/GeneCorner. This plasmid was deposited by V. Feys and Prof. Dr W. Fiers. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.