Last data update: 24 January 2024 16:39 CET
Plasmid name: pSV55 (LMBP 1833)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
psv55.gb
(View with Genome Compiler) psv55.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Simian virus 40 late promoter (SV40 late) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV54 |
| Further information: | The plasmid was constructed by opening the unique KpnI site of pSV54; after a Klenow reaction without triphosphates (exonuclease activity) and a 'classic' Klenow reaction an EcoRI linker ('GGAATTCC') was inserted. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727594.1. Other name of the plasmid is pSV532. |
| EMBL Accession number: | LT727594.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 28/11/1989 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI and PstI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by J. Tavernier(1) and Prof. Dr W. Min Jou(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Tavernier et al., Nature 301 (1983), 634-636 [PMID: 6402710] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSV55 (LMBP 1833) is available at BCCM/GeneCorner. This plasmid was deposited by J. Tavernier and Prof. Dr W. Min Jou and was published in Tavernier et al., 1983. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.