Last data update: 24 January 2024 16:39 CET
Plasmid name: pSVSPORTcHLhLTE (LMBP 3797)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p3797.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Gallus gallus hepatic lectin cDNA (HL, GeneID 396477); presequence Human lymphotoxin alpha cDNA (LTA, LT, TNFB, TNFSF1, GeneID 4049); mature sequence E-tag; C-terminal |
Promoter: | Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) Escherichia coli lac operon promoter; mutant (lacUV5) Simian virus 40 early promoter (SV40 early) |
Ribosome binding site: |
- |
Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
Parental clone: | pSV-Sport-Etag |
Further information: | The gHL-hLT fusion gene was constructed by fusion PCR and inserted as an EcoRI-NotI fragment into the EcoRI-NotI opened pSV-Sport-Etag vector, resulting in a C-terminal fusion to the E-tag. This plasmid contains the Gallus gallus (chicken) hepatic lectin membrane anchor sequence (gHL) fused to mature human lymphotoxin α cDNA (hLT); with C-terminal E-tag. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. Other name of the plasmid is pSV-SPORT-1-CHL.HLT-α-Etag. |
EMBL Accession number: | J03188, view at EMBL, GenBank, DDBJ D00102, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 31/01/2000 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | Decoster et al., J. Cell Biol. 143 (1998), 2057-2065 [PMID: 9864375] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSVSPORTcHLhLTE (LMBP 3797) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and was published in Decoster et al., 1998. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.