Last data update: 24 January 2024 16:39 CET
Plasmid name: pSVhR55-L351A (LMBP 4502)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4502.gb
(View with Genome Compiler) p4502.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human tumor necrosis factor receptor p55 cDNA (TNFR55, TNFR1, TNFRSF1A); L351A mutant |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV25S-hTNF-R55 |
| Further information: | The plasmid was derived from pSV25S-hTNF-R55 by site-directed mutagenesis of the hTNFR55 cDNA changing amino acid codon 351, located in the death domain (DD), from CTG (Leu) to GCG (Ala) and removing the unique EspI site. The plasmid contains the SV40 small t-antigen splicing signal. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727237.1. The nucleotide sequence of the hTNFR55 cDNA corresponds with the Genbank accession number M58286.1. Other name of the plasmid is SV40-lpr. |
| EMBL Accession number: | M58286.1, view at EMBL, GenBank, DDBJ LT727237.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 17/06/2002 |
| Sequence detail: | 351
hR55-wild type: 5'... CGG.CGC.CTA.GGG.CTG.AGC.GAC ... 3'
Arg Arg Leu Gly Leu Ser Arp
EspI
351
hR55-L351A: 5'... CGG.CGC.CTA.GGG.GCG.AGC.GAC ... 3'
Arg Arg Leu Gly Ala Ser Arp
^^
^: Mutated nucleotide. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindIII and PvuII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Boone et al., FEBS Lett. 441 (1998), 275-280 [PMID: 9883899] |
| Related plasmid reference: | Boone et al., J. Biol. Chem. 275 (2000), 37596-37603 [PMID: 10988295] Peppelenbosch et al., J. Immunol. 162 (1999), 837-845 [PMID: 9916706] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSVhR55-L351A (LMBP 4502) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Haegeman and was published in Boone et al., 1998. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.