Last data update: 24 January 2024 16:39 CET
Plasmid name: pSVhR55-Y331F (LMBP 4515)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4515.gb
(View with Genome Compiler) p4515.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human tumor necrosis factor receptor p55 cDNA (TNFR55, TNFR1, TNFRSF1A); Y331F mutant |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV25S-hTNF-R55 |
| Further information: | The plasmid was derived from pSV25S-hTNF-R55 by site-directed mutagenesis of the hTNFR55 cDNA changing amino acid codon 331, located in the death domain (DD), from TAC (Tyr) to TTC (Phe) and creating a second SapI restriction site. The plasmid contains the SV40 small t-antigen splicing signal. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727249.1. The nucleotide sequence of the hTNFR55 cDNA corresponds with the Genbank accession number M58286.1. |
| EMBL Accession number: | M58286.1, view at EMBL, GenBank, DDBJ LT727249.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 03/01/2006 |
| Sequence detail: | |--> death domain
| 331
hTNFR55 wild type: 5'...GAT.GAC.CCC.GCG.ACG.CTG.TAC.GCC.GTG...3'
Asp Asp Pro Ala Thr Leu Tyr Ala Val
| 331
hTNFR55-Y331F: 5'...GAT.GAC.CCC.GCG.ACG.CTC.TTC.GCC.GTG...3'
Asp Asp Pro Ala Thr Leu Phe Ala Val
* **
SapI
*: Mutated nucleotide. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, HindIII and PvuII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Vandenabeele et al., J. Immunol. 154 (1995), 2904-2913 [PMID: 7876558] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSVhR55-Y331F (LMBP 4515) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Haegeman. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.