Last data update: 24 January 2024 16:39 CET
Plasmid name: pSVhR55d243-383 (LMBP 4501)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4501.gb
(View with Genome Compiler) p4501.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human tumor necrosis factor receptor p55 cDNA (TNFR55, TNFR1, TNFRSF1A); deletion mutant |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV25S-hTNF-R55 |
| Further information: | The plasmid was derived from pSV25S-hTNF-R55 vector by deleting the N-terminal part of the death domain (codons 243-383) of the hTNFR55 cDNA. The plasmid contains the SV40 small t-antigen splicing signal. The mutant protein is named hR55Δ243-383 in Boone et al. (2000). The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727236.1. The hTNFR55 nucleotide sequence bordering the deletion has been analysed at LMBP. The nucleotide sequence of the hTNFR55 cDNA corresponds with the Genbank accession number M58286.1. |
| EMBL Accession number: | M58286.1, view at EMBL, GenBank, DDBJ LT727236.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 12/10/2005 |
| Sequence detail: | Nucleotide sequence around the fusion between the N-terminal part of the membrane-proximal region and the C-terminal part of the intracellular domain (death domain) of the human tumor necrosis factor receptor p55:
membrane-proximal region-->| |--> death domain
235 240 241 242 243 385 386 387 388 389 390
5'...ACT.AAG.CCC.CTG.GCC.CCA.AAC.CCA.AGC.TGG.CGC.GAG.GCC.ACG.CTG.GAG... 3'
Thr Lys Pro Leu Ala Pro Asn Pro Ser Trp Arg Glu Ala Thr Leu Glu
***
***: Codon created by ligation. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglII, EcoRI, PvuII and SacII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Boone et al., J. Biol. Chem. 275 (2000), 37596-37603 [PMID: 10988295] |
| Related plasmid reference: | Vandenabeele et al., J. Immunol. 154 (1995), 2904-2913 [PMID: 7876558] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSVhR55d243-383 (LMBP 4501) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Haegeman and was published in Boone et al., 2000. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.