Last data update: 24 January 2024 16:39 CET
Plasmid name: pSVhR55d327-426 (LMBP 4514)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4514.gb
(View with Genome Compiler) p4514.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human tumor necrosis factor receptor p55 cDNA (TNFR55, TNFR1, TNFRSF1A); deletion mutant E-tag; C-terminal |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pSV25S-hTNF-R55 |
| Further information: | The plasmid was derived from pSV25S-hTNF-R55 vector by deleting the death domain (codons 326-426) of the hTNFR55 cDNA. A C-terminal E-tag was added to the coding sequence of hTNFR55. The plasmid contains the SV40 small t-antigen splicing signal. The mutant protein is named hR55Δ327-426 in Boone et al. (2000). The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727248.1. The hTNFR55 nucleotide sequence bordering the deletion has been analysed at LMBP. The nucleotide sequence of the hTNFR55 cDNA corresponds with the Genbank accession number M58286.1. |
| EMBL Accession number: | M58286.1, view at EMBL, GenBank, DDBJ LT727248.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 18/10/2005 |
| Sequence detail: | Human TNF receptor p55 deletion region:
membrane-proximal region -->| |--> E-tag
320 325 | |
5'...CAG.AGC.CTA.GAC.ACT.GAT.GCG.GCC.GCA.GGT.GCG.CCG.GTG.CCG.TAT.CCG.
Gln Ser Leu Asp Thr Asp Ala Ala Ala Gly Ala Pro Ala Pro Tyr Pro
NotI
E-tag -->|
GAT.CCG.CTG.GAA.CCG.CGT.GCC.GCA.TAG.ACTGTTGAAAGTT...3'
Asp Pro Leu Glu Pro Arg Ala Ala *
*: Termination codon.
Punctuation indicates reading frame. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, HindIII and SalI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Boone et al., J. Biol. Chem. 275 (2000), 37596-37603 [PMID: 10988295] |
| Related plasmid reference: | Vandenabeele et al., J. Immunol. 154 (1995), 2904-2913 [PMID: 7876558] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSVhR55d327-426 (LMBP 4514) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Haegeman and was published in Boone et al., 2000. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.