Last data update: 24 January 2024 16:39 CET
Plasmid name: pSilent-1 (LMBP 8053)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p8053.gb
(View with Genome Compiler) p8053.txt p8053.pdf |
| Sequence analysis results Genecorner: |
NGS: d07-gc-dec2018.fasta |
| Cloned DNA: |
- |
| Promoter: | Aspergillus nidulans tryptophan C promoter (trpC) Escherichia coli lac operon promoter Phage T7 gene 10 promoter (T7g10) Phage T3 promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Aspergillus nidulans tryptophan C terminator (trpC) |
| Selection marker: | Ampicillin (amp) Hygromycin (hyg) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Ascomycete fungi |
| Parental clone: | pBluescriptIISK+ |
| Further information: | The plasmid was constructed as follows: 1) pBSTRP-PT was constructed by cloning the trpC promoter and terminator as EcoRI/ClaI and BamHI/KpnI fragments respectively, into the pBluescriptIISK+ vector, 2) The PCR amplified hygromycin resistance gene was ligated as a NotI fragment into the NotI opened pBSTRP-PT vector, resulting in vector pBSTRP-PT-H, 3) ligating a PCR amplified fragment of the M. oryzae cutinase (CUT) intron with multiple cloning sites into pBSTRP-PT-H. This plasmid is a silencing vector for ascomycete fungi. The nucleotide sequence of the pSilent-1 vector corresponds with the EMBL Nucleotide Sequence Database accession number AB303070.2, except for the changes implemented based on the NGS sequencing results. |
| EMBL Accession number: | AB303070.2, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 09/09/2019 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglI, HindIII, NotI and PvuI. This plasmid has also been fully sequenced. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr K. McCluskey(1). (1) FGSC, University of Missouri, Kansas City, USA |
| Plasmid reference: | Nakayashiki et al., Fungal Genet. Biol. 42 (2005), 275-283 [PMID: 15749047] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pSilent-1 (LMBP 8053) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr K. McCluskey and was published in Nakayashiki et al., 2005. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.